Coding

Part:BBa_K4759034:Design

Designed by: Shiyue Zhao   Group: iGEM23_Jiangnan-China   (2023-10-05)


camA-RBS2-camB


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1279
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1279
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1279
    Illegal BamHI site found at 1273
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1279
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1279
    Illegal AgeI site found at 37
    Illegal AgeI site found at 331
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We used the RBS2 sequence to express both camA and camB under the control of the same promoter


Source

camA and camB from Pseudomonas putida

References